An improved coupled test procedure for glutathione peroxidase (EC 1-11-1-9-) in blood.
نویسندگان
چکیده
An optimized coupled test system for glutathione peroxidase in red blood cells is presented. In the improved assay, tert. butyl hydroperoxide was employed as an acceptor substrate at twenty times Km(app) concentration. By aid of a batching procedure for instant separation of hemoglobin from glutathione peroxidase, it could be proved that cyanmethemoglobin in the modified procedure does not affect the spontaneous reaction rate. Further possible interference with the determination could be excluded. Glutathione peroxidase activities in red blood cells of normal adults were measured by the improved test method. A glutathione peroxidase activity in human plasma was observed.
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ورودعنوان ژورنال:
- Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 12 10 شماره
صفحات -
تاریخ انتشار 1974